primary antibodies against endogenous hnf1a protein (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
93
Structured Review
Santa Cruz Biotechnology
primary antibodies against endogenous hnf1a protein
Primary Antibodies Against Endogenous Hnf1a Protein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+endogenous+hnf1a+protein/HNF-1%CE%B1+Antibody/pm34035238-374-0-6
Average 93 stars, based on 189 article reviews
Primary Antibodies Against Endogenous Hnf1a Protein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+endogenous+hnf1a+protein/HNF-1%CE%B1+Antibody/pm34035238-374-0-6
Average 93 stars, based on 189 article reviews
primary antibodies against endogenous hnf1a protein - by Bioz Stars,
2026-09
93/100 stars
Images
Related Articles
Functional Assay:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Binding Assay:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Mutagenesis:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Sequencing:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Quantitative RT-PCR:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Flow Cytometry:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Expressing:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Derivative Assay:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Reverse Transcription:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Polymerase Chain Reaction:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). One-tailed Test:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). RNA Sequencing:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Gene Expression:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). MANN-WHITNEY:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). ChIP-sequencing:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Transfection:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Negative Control:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Western Blot:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Construct:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Immunohistochemistry:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Staining:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Immunofluorescence:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Cell Counting:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Luciferase:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Activity Assay:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). ChIP-qPCR:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). Comparison:Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Article Title: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells Article Snippet: Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).Protein lysates were quantified using the BCA Assay (Thermo Scientific), separated with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini-PROTEAN Tetra Cell system (Bio-Rad) and transferred to PVDF membranes (Bio-Rad).. Primary antibodies against endogenous HNF1A protein (Santa Cruz, sc-6548; 1:500), or actin (Sigma–Aldrich, A5441; 1:10000) were used, followed by HRP-conjugated secondary antibodies anti-goat IgG-HRP (Santa Cruz, sc-2354, 1:5000) and anti-mouse IgG-HRP (Bethyl labs, A90-516P, 1:10000).. Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific).Chemiluminescent signals were detected using Super Signal West Dura Extended Duration substrate (Thermo Scientific). |